Characterization of mesonephric development and regeneration. The aortagonad mesonephros agm region is an area derived from splanchnopleura mesoderm identified in embryonic humans, mice, and nonmammalian vertebrates such as birds and zebrafish. As fish grow, their kidneys also increase in size by the addition of new nephrons. How can i get adult zebrafish mesonephros to stay flat after its been dissected out for wax embedding. The zebrafish is a valuable vertebrate model for kidney research. The atlas of zebrafish development aims to provide a reference at the gross anatomical level for the zebrafish, identifying the major anatomical structures in samples covering the range of development from the early embryo until adulthood. Development of the zebrafish pronephric and mesonephric kidneys. It contains the dorsal aorta, genital ridges and mesonephros and lies between the notochord and the somatic mesoderm, extending from the umbilicus to the. However, our understanding of how fish mesonephric nephrons develop is very limited. The majority of previous studies focused on the zebrafish pronephros, which comprises only. A kidney related article or book chapter commonly starts with. Dissection of organs from the adult zebrafish tripti gupta 1, mary c. A guide for the laboratory use of zebrafish danio rerio.
We appreciate as much detail as possible and references as appropriate. Researchers studying embryonic kidney development in zebrafish and. This book is a comprehensive study on these findings and the only book available with such in depth coverage of the kidney. In comparison, the adult zebrafish kidney, or mesonephros, is a single, relatively. The kidney extends the length of the body cavity figures 2. In zebrafish, two nephrons are first formed as part of the embryonic kidney pronephros and hundreds are formed later to make up the adult kidney mesonephros. Development of the zebrafish pronephric and mesonephric. Like its mammalian ortholog, cdh17 is also expressed in liver and intestine in adult zebrafish. Previous studies have focused on the development of the pronephros while considerably less is known about how the mesonephros is formed.
The zebrafish is a useful genetic and embryological model system for investigating renal development, regeneration, and disease. In adult zebrafish, the mesonephros exhibits ongoing. In mammals and other amniotes, the pronephros and mesonephros. To evaluate the zebrafish system for more complex studies of kidney development and regeneration, we investigated the development and postinjury regeneration of the mesonephros in adult zebrafish. The anterior or head kidney contains lymphoid, hematopoietic, steroidogenic, and endocrine cells. The zebrafish mesonephros undergoes neonephrogenesis throughout larval and adult life, making it a novel model to study nephrogenesis and to investigate unique mechanisms of kidney regeneration.
Dissection of organs from the adult zebrafish protocol. In zebrafish, kidney development progresses through two types of kidney. The mesonephros is the second and final kidney type in zebrafish and is formed in the juvenile from progenitor cells that are labeled by the lhx1a. Live imaging kidney development in zebrafish request pdf. Please use this form to recommend updates to the information in zfin. Kidney regeneration in fish fulltext nephron experimental. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274 university of oregon, eugene, or 97403 usa. How can i get adult zebrafish mesonephros to stay flat. We have isolated a zebrafish cadherin that is orthologous to human li cadherin cdh17. The mesonephros becomes the adult kidney in the zebrafish and contains the nephrons responsible for filtering blood wastes and for salt and water uptake. Mesonephric definition of mesonephric by medical dictionary. Recipes, in the zebrafish book, chapter 10, university of oregon press. Early development of the zebrafish pronephros and analysis of. I can dissect out adult mesonephros from zebrafish but i need them to stay flat and not fold.
In adult zebrafish, the mesonephros exhibits ongoing nephrogenesis. Flow sorting of thrombocytes from the mesonephros of adult cd41. The zebrafish embryo forms an architecturally simple pronephros that has two nephrons, and these eventually become a scaffold onto which a mesonephros of several hundred nephrons is constructed. Zebrafish cdh17 is expressed exclusively in the pronephric ducts during embryogenesis, and in the mesonephros during larval development and adulthood. Mullins 1 1 department of cell and developmental biology, university of pennsylvaniaschool of medicine. In zebrafish, the mesonephric nephrons show a segmentation pattern similar to that of the pronephros, comprising 2 proximal segments pi and. Zebrafish renal development and regeneration sciencedirect. Development of the zebrafish mesonephros diep 2015. Only the pronephric and mesonephric kidneys develop in zebrafish davidson, 2011.
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